Evaluation Of A Chemical Substance - 1561 Words

Spectrophotometry is an analytical method that measures how much a chemical substance is able to absorb light by measuring the light’s intensity as it passes through a specified sample.3 Given certain wavelengths, the amount of a certain substance in a solution can be determined. The device used to measure these properties is called a spectrophotometer. This device consists of a spectrometer that produces light of any color (wavelength), and a photometer which measures the intensity of the light.2 In this experiment serial dilutions will be performed and from this, calibration curves will be produced. New solutions will be created from these dilutions and each new concentration will be found by using the concentration and volume formula†¦show more content†¦Four solutions with different concentrations were created with the B4 solution containing 10mL. The C1V1=C2V2 formula was utilized again to calculate the new concentrations and the corresponding values were recorded. Eight standard solutions were tested using a cuvette and a spectrophotometer. Two spectra were ultimately saved, one for the Red 40 and one for the Blue 1. 3mL of a standard solution was added to a cuvette and was prepared for data collection. Enough solution was added to obtain viable results. The cuvette was placed in the SpectroVis accordingly. The absorbance of the sample was recorded from 380-900nm, and the wavelength of maximum absorbency was determined along with the absorbance at the ÃŽ »max. T hese steps were repeated to determine the ÃŽ »max and absorbance for each of the eight standard solutions. The results were recorded and the spectrum was saved. Part C was performed using prepared drink samples of Kool-AidTM. The absorbance of each sample was determined at the two average ÃŽ »max wavelengths that were previously found in Part B. The recorded absorbencies were made sure to fall in the respective absorbance values of the standard solutions. RESULTS DISCUSSION In this experiment, the concepts of serial dilutions and the absorbency of light were explored and analyzed. Calibration curves were also used that consisted of the ÃŽ »max and absorbances of standards. In addition, spectrophotometry was used to determine the concentrations ofShow MoreRelatedHow And Why Safe Working Practices1121 Words   |  5 Pagesother education centres to give potential hazards and information for technician staff that use concentrated chemicals to prepare more dilute solutions for a class/lesson. In working environments they are used to produce data sheets for chemical manufacturers to give all relevant information for all of the products that they use. Details referencing what to avoid when using the substance, exposure limits and any specific hazards will also be displayed on the hazcards. 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